Exploration on Detecting Antibody Against Rabies by ELISA

Abstract:

This study is to explore the application value of enzymelinked immunosorbent assay (ELISA) in detecting the level of rabies immune antibody. We tested 290 canine sera from 10 administrative districts of Shenzhen. We use the fluorescent antibody virus neutralization test (FAVN) method as the gold standard to analyze the consistency, coincidence rate and quantitative detection effectiveness of the test results and other indicators. The results showed that the Kappa value was 0.545, and the consistency evaluation was moderately consistent. The total coincidence rate was 87.59%, and samples with a neutralizing titer of 0.512.00IU/mL had a higher probability of false negatives. In addition, the S/CO values of samples with the same neutralization titer showed a multiinterval distribution phenomenon. Therefore, this study suggests that the ELISA method can be used for preliminary screening of qualitative detection when it is applied to the actual detection of immune effect evaluation at the grassroots level, and it is not recommended to be used as a quantitative detection method.