Comparative Study on Different Freezing Methods of Mouse Oocytes and Embryos

Abstract:

This paper explores the effects of programmed freezing and vitrification on the recovery rate and developmental potential of mouse GV stage oocytes and twocell stage embryos. We compared different cryopreservation methods of mouse oocytes and early embryos to provide a reference for subsequent Avang sheep embryo cryopreservation. In this study, programmed freezing and vitrification techniques were used to freeze mouse GV stage oocytes and twocell stage embryos respectively, and culture them after recovery. The recovery rate, maturity rate and blastocyst rate after different freezing treatments were compared. The programmed freezing recovery rate (48.00% ± 5.29%) of mouse GV stage oocytes was significantly lower than the vitrification recovery rate (65.00% ± 5.00%), with statistical difference (P = 0.0147 < 0.05). The development and maturation rate of revived oocytes after programmed freezing was slightly higher than that of vitrification group, but there was no statistical significance. The recovery rate (76.00% ± 2.00%) of the mouse twocell stage embryo programmed freezing group was significantly higher than that of the vitrification group (70.00% ± 2.00%), with statistical difference (P = 0.0213 < 0.05). The rate of blastocyst blastocysts in thawed embryos developed by programmed freezing was slightly lower than that of vitrification and control group, but there was no statistical significance.